More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Anti-ATF4 (R239) Antibody
Anti-ATF4 (R239) Antibody

Anti-ATF4 (R239) Antibody

A25056
Antibodies.com
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-ATF4 (R239) Antibody
  • Delivery Days Customer
    7
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    >95%
  • Host
    Rabbit
  • Scientific Description
    Rabbit polyclonal antibody to ATF4 (R239)
  • UNSPSC
    12352203

Related products

Product group Antibodies
Everest Biotech
Goat anti-ATF4EB05247
ApplicationsWestern Blot, ELISA
TargetATF4
  • SizePrice
Product group Antibodies
Biomatik
ATF4 Polyclonal AntibodyCAC12990
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetATF4
  • SizePrice
Product group Antibodies
Assay Genie
ATF4 AntibodyCAB0201
TargetATF4
  • SizePrice
Product group Antibodies
References
GeneTex
ATF4 antibodyGTX101943
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetATF4
  • SizePrice
Western Blot analysis of Jurkat K562 cells using CREB-2 Polyclonal Antibody
Product group Antibodies
Cusabio
ATF4 AntibodyCSB-PA001783
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
TargetATF4
  • SizePrice
Product group Antibodies
RayBiotech
Anti-ATF4 Antibody144-00201
ApplicationsImmunoFluorescence, Western Blot
TargetATF4
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded Rat spleen; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with ATF4/CREB-2 Polyclonal Antibody, Unconjugated (bs-1531R) at 1:200 overnight at 4°C, DAB staining.
Product group Antibodies
References
Bioss Antibodies
ATF4 Polyclonal AntibodyBS-1531R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetATF4
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ATF4 AntibodyLS-C400594
ApplicationsELISA, ImmunoHistoChemistry
TargetATF4
  • SizePrice
Product group Antibodies
MedChem Express
Phospho-ATF4 (Ser219) AntibodyHY-P83667
TargetATF4
  • SizePrice
Figure 1. Western blot analysis of ATF4 using anti-ATF4 antibody (A00371-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF4 antigen affinity purified polyclonal antibody (Catalog # A00371-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF4 at approximately 50 kDa. The expected band size for ATF4 is at 39 kDa.
Product group Antibodies
Boster Bio
Anti-ATF4 Antibody Picoband(r)A00371-1-CARRIER-FREE
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetATF4
  • SizePrice