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Goat anti-ATF4

EB05247
Everest Biotech
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetATF4
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-ATF4 Antibody
  • Delivery Days Customer
    5
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID468
  • Target name
    ATF4
  • Target description
    activating transcription factor 4
  • Target synonyms
    cAMP response element-binding protein 2; cAMP-dependent transcription factor ATF-4; cAMP-responsive element-binding protein 2; CREB2; CREB-2; cyclic AMP-dependent transcription factor ATF-4; cyclic AMP-responsive element-binding protein 2; DNA-binding protein TAXREB67; TAXREB67; tax-responsive enhancer element B67; tax-responsive enhancer element-binding protein 67; TXREB
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001666.2; NP_877962.1. GeneIDs all Nonhuman: 11911 (mouse); 79255 (rat);. Purification: Antigen affinity purified. Names and symbols: ATF4; activating transcription factor 4 (tax-responsive enhancer element B67); CREB2; TXREB; CREB-2; TAXREB67; OTTHUMP00000199130; activating transcription factor 4; cAMP response element-binding protein 2
  • Reactivity Supplier
    Human, Mouse, Rat, Pig
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATF4 using anti-ATF4 antibody (A00371-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF4 antigen affinity purified polyclonal antibody (Catalog # A00371-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF4 at approximately 50 kDa. The expected band size for ATF4 is at 39 kDa.
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