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Anti-ATF4 [RAB-T56]

Ab01734-10.29
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence
Product group Antibodies
TargetATF4
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-ATF4 [RAB-T56]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody has been used and recommended for the following methods: FC; spiked-IP; IF.
  • Applications
    Flow Cytometry, ImmunoFluorescence
  • Applications Supplier
    FC; spiked-IP; IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RAB-T56
  • Gene ID468
  • Target name
    ATF4
  • Target description
    activating transcription factor 4
  • Target synonyms
    CREB-2, CREB2, TAXREB67, TXREB, cyclic AMP-dependent transcription factor ATF-4, DNA-binding protein TAXREB67, cAMP response element-binding protein 2, cAMP-dependent transcription factor ATF-4, cAMP-responsive element-binding protein 2, cyclic AMP-responsive element-binding protein 2, tax-responsive enhancer element B67, tax-responsive enhancer element-binding protein 67
  • Host
    Human
  • Protein IDP18848
  • Protein Name
    Cyclic AMP-dependent transcription factor ATF-4
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was obtained by recombinant antibody (rAb) phage display recognizing ATF4 protein under non-denaturing conditions.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATF4 using anti-ATF4 antibody (A00371-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SW620 whole cell lysates, Lane 2: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATF4 antigen affinity purified polyclonal antibody (Catalog # A00371-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATF4 at approximately 50 kDa. The expected band size for ATF4 is at 39 kDa.
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