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Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in granular pattern in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in granular pattern in neuronal cells.
Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in granular pattern in neuronal cells.

Anti-ATP5D Antibody

HPA002865
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetATP5F1D
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-ATP5D
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID513
  • Target name
    ATP5F1D
  • Target description
    ATP synthase F1 subunit delta
  • Target synonyms
    ATP synthase subunit delta, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit; ATP5D; F-ATPase delta subunit; MC5DN5; mitochondrial ATP synthase complex delta-subunit precusor; mitochondrial ATP synthase, delta subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30049
  • Protein Name
    ATP synthase subunit delta, mitochondrial
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Western blot All lanes: ATP5F1D antibody at 2.44microg/ml Lane 1: Mouse heart tissue Lane 2: Raji whole cell lysate Lane 3: NIH/3T3 whole cell lysate Lane 4: A549 whole cell lysate Lane 5: HepG2 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 18 kDa Observed band size: 18 kDa
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Figure 1. Western blot analysis of ATP5D/ATP5F1D using anti-ATP5D/ATP5F1D antibody (A32272-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human Raji whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat kidney tissue lysates, Lane 7: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5D/ATP5F1D antigen affinity purified polyclonal antibody (Catalog # A32272-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5D/ATP5F1D at approximately 17 kDa. The expected band size for ATP5D/ATP5F1D is at 17 kDa.
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