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Anti-ATRX [20A2]

Ab03305-10.0
Absolute Antibody
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetATRX
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-ATRX [20A2]
  • Delivery Days Customer
    7
  • Application Supplier Note
    The antibody 20A2 and the commercial antibody TS1 were used to stain pancreatic neuroendocrine and cerebral gel tumors in immunohistochemistry. The results of the two antibodies were comparable and the staining intensity of the antibody 20A2 was higher than the commercial antibody (CN112940133A).
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    IHC; WB; ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    20A2
  • Gene ID546
  • Target name
    ATRX
  • Target description
    ATRX chromatin remodeler
  • Target synonyms
    JMS, MRX52, RAD54, RAD54L, XH2, XNP, ZNF-HX, transcriptional regulator ATRX, ATP-dependent helicase ATRX, X-linked helicase II, X-linked nuclear protein
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP46100
  • Protein Name
    Transcriptional regulator ATRX
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format for improved compatibility with existing reagents assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was generated by immunizing a mouse with the recombinant ATRX protein.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATRX using anti-ATRX antibody (A00203-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A54 whole cell lysates, Lane 2: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATRX antigen affinity purified polyclonal antibody (Catalog # A00203-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATRX at approximately 282KD. The expected band size for ATRX is at 282KD.
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Lane 1: Mouse Breast tissue lysates; Lane 2: Rat Breast tissue lysates probed with ATRX Polyclonal Antibody, Unconjugated (bs-24302R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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