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ProductsBack/ATRX Polyclonal Antibody
Lane 1: Mouse Breast tissue lysates; Lane 2: Rat Breast tissue lysates probed with ATRX Polyclonal Antibody, Unconjugated (bs-24302R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Breast tissue lysates; Lane 2: Rat Breast tissue lysates probed with ATRX Polyclonal Antibody, Unconjugated (bs-24302R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
Lane 1: Mouse Breast tissue lysates; Lane 2: Rat Breast tissue lysates probed with ATRX Polyclonal Antibody, Unconjugated (bs-24302R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.

ATRX Polyclonal Antibody

BS-24302R
Bioss
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetATRX
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Overview

  • Supplier
    Bioss
  • Product Name
    ATRX Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID546
  • Target name
    ATRX
  • Target description
    ATRX chromatin remodeler
  • Target synonyms
    JMS, MRX52, RAD54, RAD54L, XH2, XNP, ZNF-HX, transcriptional regulator ATRX, ATP-dependent helicase ATRX, X-linked helicase II, X-linked nuclear protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP46100
  • Protein Name
    Transcriptional regulator ATRX
  • Reactivity
    Bovine, Canine, Chicken, Equine, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of ATRX using anti-ATRX antibody (A00203-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A54 whole cell lysates, Lane 2: human Hela whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATRX antigen affinity purified polyclonal antibody (Catalog # A00203-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATRX at approximately 282KD. The expected band size for ATRX is at 282KD.
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