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ProductsBack/Anti-B7-H3 [A3]

Anti-B7-H3 [A3]

Ab02432-10.0
Absolute Antibody
ApplicationsELISA
Product group Antibodies
TargetCD276
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-B7-H3 [A3]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody binds human B7-H3 with a strong affinity of 3.16 pM. It positively binds human B7-H3 in an ELISA. This antibody was also capable of binding MDA-MB-231 cells that highly express B7-H3 (WO2018161872A1).
  • Applications
    ELISA
  • Applications Supplier
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    A3
  • Gene ID80381
  • Target name
    CD276
  • Target description
    CD276 molecule
  • Target synonyms
    4Ig-B7-H3, B7-H3, B7H3, B7RP-2, CD276 antigen, B7 homolog 3, costimulatory molecule
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDQ5ZPR3
  • Protein Name
    CD276 antigen
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was generated by immunizing BALB/c mice with recombinant human B7-H3.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD276 using anti-CD276 antibody (A02220-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD276 antigen affinity purified polyclonal antibody (Catalog # A02220-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD276 at approximately 90-100 kDa. The expected band size for CD276 is at 100 kDa.
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