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Western Blot analysis of 293 cells using CD276 Polyclonal Antibody
Western Blot analysis of 293 cells using CD276 Polyclonal Antibody
Western Blot analysis of 293 cells using CD276 Polyclonal Antibody

CD276 Antibody

CSB-PA005320
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetCD276
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Overview

  • Supplier
    Cusabio
  • Product Name
    CD276 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID80381
  • Target name
    CD276
  • Target description
    CD276 molecule
  • Target synonyms
    4Ig-B7-H3; B7 homolog 3; B7H3; B7-H3; B7RP-2; CD276 antigen; costimulatory molecule
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5ZPR3
  • Protein Name
    CD276 antigen
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Lane 1:JAR cell lysates;Lane 2: MCF-7 cell lysates probed with CD276 Polyclonal Antibody, Unconjugated (bs-11019R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Figure 1. Western blot analysis of CD276 using anti-CD276 antibody (A02220-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD276 antigen affinity purified polyclonal antibody (Catalog # A02220-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD276 at approximately 90-100 kDa. The expected band size for CD276 is at 100 kDa.
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