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ProductsBack/CD276 antibody [6A1]
FACS analysis of PC-3 cells using GTX82773 CD276 antibody [6A1]. Green : CD276 Purple : negative control
FACS analysis of PC-3 cells using GTX82773 CD276 antibody [6A1]. Green : CD276 Purple : negative control
FACS analysis of PC-3 cells using GTX82773 CD276 antibody [6A1]. Green : CD276 Purple : negative control

CD276 antibody [6A1]

GTX82773
GeneTex
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCD276
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD276 antibody [6A1]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    6A1
  • Conjugate
    Unconjugated
  • Gene ID80381
  • Target name
    CD276
  • Target description
    CD276 molecule
  • Target synonyms
    4Ig-B7-H3; B7 homolog 3; B7H3; B7-H3; B7RP-2; CD276 antigen; costimulatory molecule
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ5ZPR3
  • Protein Name
    CD276 antigen
  • Scientific Description
    The protein encoded by this gene belongs to the immunoglobulin superfamily, and thought to participate in the regulation of T-cell-mediated immune response. Studies show that while the transcript of this gene is ubiquitously expressed in normal tissues and solid tumors, the protein is preferentially expressed only in tumor tissues. Additionally, it was observed that the 3 UTR of this transcript contains a target site for miR29 microRNA, and there is an inverse correlation between the expression of this protein and miR29 levels, suggesting regulation of expression of this gene product by miR29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD276 using anti-CD276 antibody (A02220-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human U2OS whole cell lysates, Lane 2: human PC-3 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD276 antigen affinity purified polyclonal antibody (Catalog # A02220-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD276 at approximately 90-100 kDa. The expected band size for CD276 is at 100 kDa.
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