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Figure 1. Western blot analysis of Bax using anti-Bax antibody (PA1013-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Testis Tissue Lysate Lane 2: Rat Kidney Tissue Lysate Lane 3: Rat Brain Tissue Lysate Lane 4: Rat Ovary Tissue Lysate Lane 5: HELA Cell Lysate Lane 6: MM231 Cell Lysate Lane 7: A549 Cell Lysate Lane 8: JURKAT Cell Lysate Lane 9: Human Placenta Tissue Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bax antigen affinity purified polyclonal antibody (Catalog # PA1013-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bax at approximately 21&28KD. The expected band size for Bax is at 21KD.
Figure 1. Western blot analysis of Bax using anti-Bax antibody (PA1013-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Testis Tissue Lysate Lane 2: Rat Kidney Tissue Lysate Lane 3: Rat Brain Tissue Lysate Lane 4: Rat Ovary Tissue Lysate Lane 5: HELA Cell Lysate Lane 6: MM231 Cell Lysate Lane 7: A549 Cell Lysate Lane 8: JURKAT Cell Lysate Lane 9: Human Placenta Tissue Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bax antigen affinity purified polyclonal antibody (Catalog # PA1013-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bax at approximately 21&28KD. The expected band size for Bax is at 21KD.
Figure 1. Western blot analysis of Bax using anti-Bax antibody (PA1013-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Testis Tissue Lysate Lane 2: Rat Kidney Tissue Lysate Lane 3: Rat Brain Tissue Lysate Lane 4: Rat Ovary Tissue Lysate Lane 5: HELA Cell Lysate Lane 6: MM231 Cell Lysate Lane 7: A549 Cell Lysate Lane 8: JURKAT Cell Lysate Lane 9: Human Placenta Tissue Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bax antigen affinity purified polyclonal antibody (Catalog # PA1013-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Bax at approximately 21&28KD. The expected band size for Bax is at 21KD.

Anti-Apoptosis regulator BAX Bax Antibody Picoband(r)

PA1013-1
Boster Bio
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHamster, Human, Mouse, Rat
TargetBAX
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-Bax Antibody
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID581
  • Target name
    BAX
  • Target description
    BCL2 associated X, apoptosis regulator
  • Target synonyms
    apoptosis regulator BAX; Baxdelta2G9; Baxdelta2G9omega; Baxdelta2omega; BCL2 associated X protein; BCL2-associated X protein omega; BCL2L4; bcl2-L-4; bcl-2-like protein 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ07812
  • Protein Name
    Apoptosis regulator BAX
  • Scientific Description
    Boster Bio Anti-Apoptosis regulator BAX Bax Antibody catalog # PA1013-1. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Hamster, Human, Mouse, Rat
  • Reactivity Supplier
    Human, Mouse, Rat, Hamster
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

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  • Circ_0002331 Interacts with ELAVL1 to Improve ox-LDL-Induced Vascular Endothelial Cell Dysfunction via Regulating CCND2 mRNA Stability.
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  • Mitochondrial Fission in Nickel Nanoparticle-Induced Reproductive Toxicity: An In Vitro GC-1 Cell Study.
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  • Declined circular RNA mitofusin 2 constrains the deterioration of Wilms tumor via modulating microRNA-372-3p/transforming growth factor-beta receptor type 2 axis.
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  • Adiponectin attenuates H2O2-induced apoptosis in chicken skeletal myoblasts through the lysosomal-mitochondrial axis.
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