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Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in tubular cells.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in tubular cells.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in tubular cells.

Anti-BCAR3 Antibody

HPA014858
Atlas Antibodies
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
TargetBCAR3
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BCAR3
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8412
  • Target name
    BCAR3
  • Target description
    BCAR3 adaptor protein, NSP family member
  • Target synonyms
    AND-34; BCAR3, NSP family adaptor protein; breast cancer anti-estrogen resistance 3; breast cancer antiestrogen resistance 3 protein; breast cancer anti-estrogen resistance protein 3; dJ1033H22.2 (breast cancer anti-estrogen resistance 3); epididymis secretory sperm binding protein; MIG7; mig-7; migration inducting gene-7; novel SH2-containing protein 2; NSP2; SH2 domain-containing protein 3B; SH2D3B
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO75815
  • Protein Name
    Breast cancer anti-estrogen resistance protein 3
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BCAR3 using anti-BCAR3 antibody (PB9846). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCAR3 antigen affinity purified polyclonal antibody (Catalog # PB9846) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCAR3 at approximately 93 kDa. The expected band size for BCAR3 is at 93 kDa.
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