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BCAR3 Antibody (aa25-75)

LS-C288459
LifeSpan BioSciences
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
TargetBCAR3
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BCAR3 Antibody (aa25-75)
  • Delivery Days Customer
    23
  • Applications
    ImmunoPrecipitation, Western Blot
  • Applications Supplier
    IP (2 - 5 µg/mg lysate), WB (1:2000 - 1:10000)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.2 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID8412
  • Target name
    BCAR3
  • Target description
    BCAR3 adaptor protein, NSP family member
  • Target synonyms
    AND-34; BCAR3, NSP family adaptor protein; breast cancer anti-estrogen resistance 3; breast cancer antiestrogen resistance 3 protein; breast cancer anti-estrogen resistance protein 3; dJ1033H22.2 (breast cancer anti-estrogen resistance 3); epididymis secretory sperm binding protein; MIG7; mig-7; migration inducting gene-7; novel SH2-containing protein 2; NSP2; SH2 domain-containing protein 3B; SH2D3B
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BCAR3 using anti-BCAR3 antibody (PB9846). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: HEPG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCAR3 antigen affinity purified polyclonal antibody (Catalog # PB9846) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCAR3 at approximately 93 kDa. The expected band size for BCAR3 is at 93 kDa.
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