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Anti-beta Tubulin Antibody

102-25104
RayBiotech
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot
Product group Antibodies
TargetTUBB2A
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-beta Tubulin Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    RB55943
  • Gene ID7280
  • Target name
    TUBB2A
  • Target description
    tubulin beta 2A class IIa
  • Target synonyms
    CDCBM5; class IIa beta-tubulin; TUBB; TUBB2; tubulin beta-2A chain; tubulin, beta 2A; tubulin, beta polypeptide 2
  • Host
    Rabbit
  • Protein IDO17449
  • Protein Name
    Tubulin beta-1 chain
  • Scientific Description
    Rabbit Anti-beta Tubulin Antibody, 200 microl
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1: Immunoblot analysis of protein glutamylation with anti-beta-Tubulin (beta-monoE), pAb (IN115) (Prod. No. AG-25B-0039). Method: HEK-293T cells are grown in standard culture conditions, transfected with plasmids expressing the different glutamylases and are run on a 10% SDS-PAGE (specific protocol to separate alpha- and beta-tubulin (Magiera & Janke, 2013)). Tubulin purified from pork brain was used as a positive control for glutamylated tubulin. The proteins are transferred to a nitrocellulose membrane and detected by standard immunoblot protocol using the beta-monoE (1:5,000) in PBS containing 0.1% Tween-20 for washing steps and 2.5% fat free milk for antibody incubation. In untransfected HEK-293T cell extracts, no tubulin is detected, even on high exposure. After expression of TTLL4, predominantly beta-tubulin is detected with beta-monoE, but the antibody also faintly detects the heterogeneous mixture of different, yet unidentified substrates modified by TTLL4. In cells expressing TTLL5 and TTLL6, the beta-monoE detects only beta-tubulin, while expression of TTLL7 generates beta-monoE-positive alpha- and beta-tubulin. In the purified brain tubulin, the antibody specifically detects glutamylation of beta-tubulin. Picture courtesy of Sudarshan Gadadhar, Shreyangi Chakraborty, Mariya Genova and Carsten Janke, Institut Curie, Orsay.
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