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TUBB Monoclonal Antibody

MACO0666
Assay Genie
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rabbit, Rat
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Overview

  • Supplier
    Assay Genie
  • Product Name
    TUBB Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    ELISA, WB, IHC, IF, FC, IP
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    16E11D4
  • Conjugate
    Unconjugated
  • Host
    Mouse
  • Protein IDP04350
  • Protein Name
    Tubulin beta-4A chain
  • Scientific Description
    TUBB Monoclonal Antibody is a premium monoclonal that offers outstanding performance and reliability for demanding research applications. Rigorously validated for ELISA, WB, IHC, IF, FC, IP, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human, Rat, Rabbit, Mouse samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 50ul format to meet your experimental needs. For optimal performance, store at Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Mouse
  • Reactivity
    Human, Mouse, Rabbit, Rat
  • Reactivity Supplier
    Human, Rat, Rabbit, Mouse
  • Storage Instruction
    -80°C
  • UNSPSC
    41116161

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Figure 1: Immunoblot analysis of protein glutamylation with anti-beta-Tubulin (beta-monoE), pAb (IN115) (Prod. No. AG-25B-0039). Method: HEK-293T cells are grown in standard culture conditions, transfected with plasmids expressing the different glutamylases and are run on a 10% SDS-PAGE (specific protocol to separate alpha- and beta-tubulin (Magiera & Janke, 2013)). Tubulin purified from pork brain was used as a positive control for glutamylated tubulin. The proteins are transferred to a nitrocellulose membrane and detected by standard immunoblot protocol using the beta-monoE (1:5,000) in PBS containing 0.1% Tween-20 for washing steps and 2.5% fat free milk for antibody incubation. In untransfected HEK-293T cell extracts, no tubulin is detected, even on high exposure. After expression of TTLL4, predominantly beta-tubulin is detected with beta-monoE, but the antibody also faintly detects the heterogeneous mixture of different, yet unidentified substrates modified by TTLL4. In cells expressing TTLL5 and TTLL6, the beta-monoE detects only beta-tubulin, while expression of TTLL7 generates beta-monoE-positive alpha- and beta-tubulin. In the purified brain tubulin, the antibody specifically detects glutamylation of beta-tubulin. Picture courtesy of Sudarshan Gadadhar, Shreyangi Chakraborty, Mariya Genova and Carsten Janke, Institut Curie, Orsay.
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