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ProductsBack/Anti-Beta Tubulin type VI [AA16 (6A10)]

Anti-Beta Tubulin type VI [AA16 (6A10)]

Ab02684-10.0
Absolute Antibody
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetTUBB
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-Beta Tubulin type VI [AA16 (6A10)]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody is recommended for WB and IF.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    WB; IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AA16 (6A10)
  • Gene ID203068
  • Target name
    TUBB
  • Target description
    tubulin beta class I
  • Target synonyms
    CDCBM6, CSCSC1, M40, OK/SW-cl.56, TUBB1, TUBB5, tubulin beta chain, beta Ib tubulin, epididymis secretory sperm binding protein, tubulin beta-1 chain, tubulin beta-5 chain, tubulin, beta polypeptide
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP07437
  • Protein Name
    Tubulin beta chain
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human, Mammals
  • Reactivity Supplier Note
    This antibody was raised by immunizing a mouse with the c-terminus (DEEVTEEAEMEPEDKGH) of human beta tubulin type VI.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Beta Tubulin using anti-Beta Tubulin antibody (A01857-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: chicken heart tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Beta Tubulin antigen affinity purified polyclonal antibody (Catalog # A01857-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Beta Tubulin at approximately 50 kDa. The expected band size for Beta Tubulin is at 50 kDa.
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