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Immunohistochemical staining of human lymph node shows cytoplasmic positivity in germinal center.
Immunohistochemical staining of human lymph node shows cytoplasmic positivity in germinal center.
Immunohistochemical staining of human lymph node shows cytoplasmic positivity in germinal center.

Anti-TUBB Antibody

HPA046280
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
DownloadDatasheet
DownloadMSDS
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetTUBB
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-TUBB
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID203068
  • Target name
    TUBB
  • Target description
    tubulin beta class I
  • Target synonyms
    beta Ib tubulin; CDCBM6; CSCSC1; epididymis secretory sperm binding protein; M40; OK/SW-cl.56; TUBB1; TUBB5; tubulin beta chain; tubulin beta-1 chain; tubulin beta-5 chain; tubulin, beta polypeptide
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07437
  • Protein Name
    Tubulin beta chain
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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RSC96 cells probed with Tubulin-beta Polyclonal Antibody, Unconjugated (bs-0210R) at 1:20 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
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Figure 1. Western blot analysis of Beta Tubulin using anti-Beta Tubulin antibody (A01857-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SiHa whole cell lysates, Lane 2: human 293T whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: monkey COS-7 whole cell lysates, Lane 5: chicken heart tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse brain tissue lysates, Lane 9: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Beta Tubulin antigen affinity purified polyclonal antibody (Catalog # A01857-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Beta Tubulin at approximately 50 kDa. The expected band size for Beta Tubulin is at 50 kDa.
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