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Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.
Immunohistochemical staining of human kidney shows moderate cytoplasmic positivity in cells in tubules.

Anti-BIRC2 Antibody

HPA005513
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Rat
TargetBIRC2
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-BIRC2
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID329
  • Target name
    BIRC2
  • Target description
    baculoviral IAP repeat containing 2
  • Target synonyms
    API1, HIAP2, Hiap-2, MIHB, RNF48, c-IAP1, cIAP1, baculoviral IAP repeat-containing protein 2, IAP homolog B, IAP-2, NFR2-TRAF signalling complex protein, RING finger protein 48, RING-type E3 ubiquitin transferase BIRC2, TNFR2-TRAF-signaling complex protein 2, apoptosis inhibitor 1, cellular inhibitor of apoptosis 1, inhibitor of apoptosis protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13490
  • Protein Name
    Baculoviral IAP repeat-containing protein 2
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of cIAP1 using anti-cIAP1 antibody (A01700-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human COLO-320 whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: rat thymus tissue lysates, Lane 8: mouse thymus tissue lysates, Lane 9: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-cIAP1 antigen affinity purified polyclonal antibody (Catalog # A01700-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for cIAP1 at approximately 70KD. The expected band size for cIAP1 is at 70KD.
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