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ProductsBack/BIRC2 / cIAP1 Antibody (aa1-300)

BIRC2 / cIAP1 Antibody (aa1-300)

LS-C746764
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot
Product group Antibodies
TargetBIRC2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    BIRC2 / cIAP1 Antibody (aa1-300)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 64kDa/69kDa, while the observed MW by Western blot was 69kDa.
  • Applications
    ImmunoFluorescence, Western Blot
  • Applications Supplier
    IF (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 64kDa/69kDa, while the observed MW by Western blot was 69kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    2.44 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID329
  • Target name
    BIRC2
  • Target description
    baculoviral IAP repeat containing 2
  • Target synonyms
    API1, HIAP2, Hiap-2, MIHB, RNF48, c-IAP1, cIAP1, baculoviral IAP repeat-containing protein 2, IAP homolog B, IAP-2, NFR2-TRAF signalling complex protein, RING finger protein 48, RING-type E3 ubiquitin transferase BIRC2, TNFR2-TRAF-signaling complex protein 2, apoptosis inhibitor 1, cellular inhibitor of apoptosis 1, inhibitor of apoptosis protein 2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of cIAP1 using anti-cIAP1 antibody (A01700-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human COLO-320 whole cell lysates, Lane 5: human U-87MG whole cell lysates, Lane 6: human A549 whole cell lysates, Lane 7: rat thymus tissue lysates, Lane 8: mouse thymus tissue lysates, Lane 9: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-cIAP1 antigen affinity purified polyclonal antibody (Catalog # A01700-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for cIAP1 at approximately 70KD. The expected band size for cIAP1 is at 70KD.
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