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Western blot (WB) analysis of BLK (phospho-Y389) polyclonal antibody at 1:500 dilution Lane1:COS-7whole cell lysate(40ug) Lane2:MCF-7whole cell lysate(40ug) Lane3:CT26 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug)
Western blot (WB) analysis of BLK (phospho-Y389) polyclonal antibody at 1:500 dilution Lane1:COS-7whole cell lysate(40ug) Lane2:MCF-7whole cell lysate(40ug) Lane3:CT26 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug)
Western blot (WB) analysis of BLK (phospho-Y389) polyclonal antibody at 1:500 dilution Lane1:COS-7whole cell lysate(40ug) Lane2:MCF-7whole cell lysate(40ug) Lane3:CT26 whole cell lysate(40ug) Lane4:PC12 whole cell lysate(40ug)

Anti-BLK (phospho-Y389) Antibody

A01539Y389
Boster Bio
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetBLK
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-BLK (phospho-Y389) Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Gene ID640
  • Target name
    BLK
  • Target description
    BLK proto-oncogene, Src family tyrosine kinase
  • Target synonyms
    MODY11, tyrosine-protein kinase Blk, B lymphoid tyrosine kinase, BLK nonreceptor tyrosine kinase, b lymphocyte kinase, p55-Blk
  • Host
    Rabbit
  • Protein IDP51451
  • Protein Name
    Tyrosine-protein kinase Blk
  • Scientific Description
    Boster Bio Anti-BLK (phospho-Y389) Antibody catalog # A01539Y389. Tested in WB applications. This antibody reacts with Human,Mouse,Rat.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of BLK using anti-BLK antibody (A01539-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BLK antigen affinity purified polyclonal antibody (Catalog # A01539-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BLK at approximately 60 kDa. The expected band size for BLK is at 60 kDa.
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