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Immunohistochemical staining of human cervix, uterine shows no positivity in squamous epithelial cells as expected.
Immunohistochemical staining of human cervix, uterine shows no positivity in squamous epithelial cells as expected.
Immunohistochemical staining of human cervix, uterine shows no positivity in squamous epithelial cells as expected.

Anti-CACNA1S Antibody

HPA048892
Atlas Antibodies
ApplicationsImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCACNA1S
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CACNA1S
  • Delivery Days Customer
    4
  • Applications
    ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID779
  • Target name
    CACNA1S
  • Target description
    calcium voltage-gated channel subunit alpha1 S
  • Target synonyms
    CACNL1A3, CCHL1A3, CMYO18, CMYP18, Cav1.1, DHPRM, HOKPP, HOKPP1, MHS5, TTPP1, hypoPP, voltage-dependent L-type calcium channel subunit alpha-1S, calcium channel, voltage-dependent, L type, alpha 1S subunit, dihydropyridine receptor alpha 1 subunit, dihydropyridine-sensitive L-type calcium channel alpha-1 subunit, voltage-gated calcium channel subunit alpha Cav1.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13698
  • Protein Name
    Voltage-dependent L-type calcium channel subunit alpha-1S
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CACNA1S using anti-CACNA1S antibody (A03006-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: monkey skeletal muscle tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CACNA1S antigen affinity purified polyclonal antibody (Catalog # A03006-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CACNA1S at approximately 220KD. The expected band size for CACNA1S is at 220KD.
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