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Paraformaldehyde-fixed, paraffin embedded Mouse muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CACNA1S Polyclonal Antibody, Unconjugated (bs-9925R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CACNA1S Polyclonal Antibody, Unconjugated (bs-9925R) at 1:200 overnight at 4°C, DAB staining.
Paraformaldehyde-fixed, paraffin embedded Mouse muscle; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with CACNA1S Polyclonal Antibody, Unconjugated (bs-9925R) at 1:200 overnight at 4°C, DAB staining.

CACNA1S Polyclonal Antibody

BS-9925R
Bioss Antibodies
ApplicationsImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCACNA1S
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CACNA1S Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA(1:500-1000), IHC-P(1:200-400), IF(IHC-P)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID779
  • Target name
    CACNA1S
  • Target description
    calcium voltage-gated channel subunit alpha1 S
  • Target synonyms
    CACNL1A3; calcium channel, voltage-dependent, L type, alpha 1S subunit; Cav1.1; CCHL1A3; dihydropyridine receptor alpha 1 subunit; dihydropyridine-sensitive L-type calcium channel alpha-1 subunit; HOKPP; HOKPP1; hypoPP; MHS5; TTPP1; voltage-dependent L-type calcium channel subunit alpha-1S; voltage-gated calcium channel subunit alpha Cav1.1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13698
  • Protein Name
    Voltage-dependent L-type calcium channel subunit alpha-1S
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Rhesus rotavirus VP6 regulates ERK-dependent calcium influx in cholangiocytes. Lobeck I et al., 2016 Dec, Virology
    Read more

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Figure 1. Western blot analysis of CACNA1S using anti-CACNA1S antibody (A03006-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: monkey skeletal muscle tissue lysates, Lane 2: rat skeletal muscle tissue lysates, Lane 3: mouse skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CACNA1S antigen affinity purified polyclonal antibody (Catalog # A03006-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CACNA1S at approximately 220KD. The expected band size for CACNA1S is at 220KD.
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