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Anti-Calnexin Antibody

Anti-Calnexin Antibody

A94879
Antibodies.com
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
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Overview

  • Supplier
    Antibodies.com
  • Product Name
    Anti-Calnexin Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Rabbit polyclonal antibody to Calnexin.
  • Reactivity
    Human, Mouse, Rat
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Calnexin/CANX using anti-Calnexin/CANX antibody (A03372-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calnexin/CANX antigen affinity purified polyclonal antibody (Catalog # A03372-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calnexin/CANX at approximately 97 kDa. The expected band size for Calnexin/CANX is at 97 kDa.
Product group Antibodies
Boster Bio
Anti-Calnexin/CANX Antibody Picoband(r)A03372-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetCANX
  • SizePrice
Product group Antibodies
RayBiotech
Anti-CANX Antibody144-63846
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCANX
  • SizePrice
Western Blot analysis of COLO205 cells using Calnexin Polyclonal Antibody
Product group Antibodies
Cusabio
CANX AntibodyCSB-PA001175
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCANX
  • SizePrice
Product group Antibodies
References
Everest Biotech
Goat anti-CalnexinEB09525
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityBovine, Canine, Human, Mouse, Porcine, Rat
TargetCANX
  • SizePrice
Product group Antibodies
Biomatik
CANX Polyclonal AntibodyCAC14640
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetCANX
  • SizePrice
Product group Antibodies
References
Bioss
Calnexin Polyclonal AntibodyBS-1693R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Chicken, Equine, Human, Mouse, Rabbit, Rat
TargetCANX
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
CANX / Calnexin AntibodyLS-C401473
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCANX
  • SizePrice
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Product group Antibodies
GeneTex
Calnexin antibody [N3C2], InternalGTX101676
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetCANX
  • SizePrice
Immunohistochemical staining of human cerebral cortex, placenta, testis and tonsil using Anti-CANX antibody HPA009433 (A) shows similar protein distribution across tissues to independent antibody HPA009696 (B).
Product group Antibodies
Atlas Antibodies
Anti-CANX AntibodyHPA009433
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCANX
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Product group Antibodies
MedChem Express
Calnexin Antibody (YA573, Rabbit)HY-P80041
TargetCANX
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