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ProductsBack/Goat anti-Calnexin

Goat anti-Calnexin

EB09525
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetCANX
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-Calnexin Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum (ER) of NIH3T3 cells. Recommended concentration: 10microg/ml.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    Pep-ELISA, WB, IHC, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID821
  • Target name
    CANX
  • Target description
    calnexin
  • Target synonyms
    CNX, IP90, P90, calnexin, epididymis secretory sperm binding protein, major histocompatibility complex class I antigen-binding protein p88
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_001737.1. GeneIDs all Nonhuman: 12330 (mouse); 29144 (rat);. Purification: Antigen affinity purified. Names and symbols: CANX; calnexin; CNX; FLJ26570; IP90; P90; major histocompatibility complex class I antigen-binding protein p88
  • Reactivity Supplier
    Human, Mouse, Rat, Dog, Pig, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Sikorski K, Mehta A, Inngjerdingen M, et al. A high-throughput pipeline for validation of antibodies. Nat Methods. 2018,15(11):909-912. doi: 10.1038/s41592-018-0179-8
    Read this paper

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Figure 1. Western blot analysis of Calnexin/CANX using anti-Calnexin/CANX antibody (A03372-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calnexin/CANX antigen affinity purified polyclonal antibody (Catalog # A03372-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calnexin/CANX at approximately 97 kDa. The expected band size for Calnexin/CANX is at 97 kDa.
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