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Anti-CALR [SAIC-16D-6B9]

Ab00309-1.1
Absolute Antibody
ApplicationsMass Spectrometry, Western Blot, ELISA
Product group Antibodies
TargetCALR
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CALR [SAIC-16D-6B9]
  • Delivery Days Customer
    7
  • Application Supplier Note
    Original data characterizing this antibody may be found here .
  • Applications
    Mass Spectrometry, Western Blot, ELISA
  • Applications Supplier
    ELISA; WB; immunoMRM
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    SAIC-16D-6B9
  • Gene ID811
  • Target name
    CALR
  • Target description
    calreticulin
  • Target synonyms
    calregulin; calreticulin; cC1qR; CRP55; CRT; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; grp60; HACBP; HEL-S-99n; RO; Sicca syndrome antigen A (autoantigen Ro; calreticulin); SSA
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP27797
  • Protein Name
    Calreticulin
  • Scientific Description
    This is a chimeric antibody created for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    Peptide "GLQTSQDAR", derived from the calcium-binding protein chaperone Calreticulin, conjugated to KLH.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CALR using anti-CALR antibody (A00894-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CALR antigen affinity purified polyclonal antibody (Catalog # A00894-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CALR at approximately 60KD. The expected band size for CALR is at 48KD.
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