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Western Blot analysis of COS7 cells using Calregulin Polyclonal Antibody
Western Blot analysis of COS7 cells using Calregulin Polyclonal Antibody
Western Blot analysis of COS7 cells using Calregulin Polyclonal Antibody

CALR Antibody

CSB-PA001196
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetCALR
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Overview

  • Supplier
    Cusabio
  • Product Name
    CALR Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID811
  • Target name
    CALR
  • Target description
    calreticulin
  • Target synonyms
    calregulin; calreticulin; cC1qR; CRP55; CRT; endoplasmic reticulum resident protein 60; epididymis secretory sperm binding protein Li 99n; ERp60; grp60; HACBP; HEL-S-99n; RO; Sicca syndrome antigen A (autoantigen Ro; calreticulin); SSA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27797
  • Protein Name
    Calreticulin
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CALR using anti-CALR antibody (A00894-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human U-937 whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: human PC-3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CALR antigen affinity purified polyclonal antibody (Catalog # A00894-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CALR at approximately 60KD. The expected band size for CALR is at 48KD.
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