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Anti-CAMK2D Antibody

R1305-2
HUABIO
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCAMK2D
100 ul
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Overview

  • Supplier
    HUABIO
  • Product Name
    Anti-CAMK2D Antibody
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB,IF-Cell,IHC-P,FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID817
  • Target name
    CAMK2D
  • Target description
    calcium/calmodulin dependent protein kinase II delta
  • Target synonyms
    CAMKD, calcium/calmodulin-dependent protein kinase type II subunit delta, CaM kinase II delta subunit, CaM-kinase II delta chain, CaMK-II delta subunit, calcium/calmodulin-dependent protein kinase (CaM kinase) II delta, calcium/calmodulin-dependent protein kinase type II delta chain
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ13557
  • Protein Name
    Calcium/calmodulin-dependent protein kinase type II subunit delta
  • Scientific Description
    Calcium/calmodulin-dependent protein kinase involved in the regulation of Ca2+ homeostatis and excitation-contraction coupling (ECC) in heart by targeting ion channels, transporters and accessory proteins involved in Ca2+ influx into the myocyte, Ca2+ release from the sarcoplasmic reticulum (SR), SR Ca2+ uptake and Na+ and K+ channel transport. CAMK2 is composed of 4 different chains: alpha (CAMK2A), beta (CAMK2B), gamma (CAMK2G), and delta (CAMK2D). The different isoforms assemble into homo- or heteromultimeric holoenzymes composed of 12 subunits with two hexameric rings stacked one on top of the other.
  • Reactivity
    Human, Mouse
  • Reactivity Supplier
    Human,Mouse
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CaMKII delta/CAMK2D using anti-CaMKII delta/CAMK2D antibody (M02611). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HUVEC whole cell lysates, Lane 4: human A549 whole cell lysates, Lane 5: rat C6 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CaMKII delta/CAMK2D antigen affinity purified monoclonal antibody (M02611) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CaMKII delta/CAMK2D at approximately 50 kDa. The expected band size for CaMKII delta/CAMK2D is at 56 kDa.
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