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Immunohistochemical staining of human cerebral cortex, placenta, testis and tonsil using Anti-CANX antibody HPA009696 (A) shows similar protein distribution across tissues to independent antibody HPA009433 (B).
Immunohistochemical staining of human cerebral cortex, placenta, testis and tonsil using Anti-CANX antibody HPA009696 (A) shows similar protein distribution across tissues to independent antibody HPA009433 (B).
Immunohistochemical staining of human cerebral cortex, placenta, testis and tonsil using Anti-CANX antibody HPA009696 (A) shows similar protein distribution across tissues to independent antibody HPA009433 (B).

Anti-CANX Antibody

HPA009696
Atlas Antibodies
ApplicationsImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCANX
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CANX Antibody
  • Delivery Days Customer
    4
  • Applications
    ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID821
  • Target name
    CANX
  • Target description
    calnexin
  • Target synonyms
    CNX, IP90, P90, calnexin, epididymis secretory sperm binding protein, major histocompatibility complex class I antigen-binding protein p88
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP27824
  • Protein Name
    Calnexin
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of Calnexin/CANX using anti-Calnexin/CANX antibody (A03372-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calnexin/CANX antigen affinity purified polyclonal antibody (Catalog # A03372-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calnexin/CANX at approximately 97 kDa. The expected band size for Calnexin/CANX is at 97 kDa.
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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Calnexin antibody [N3C2], Internal (GTX101676) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunohistochemical staining of human cerebral cortex, placenta, testis and tonsil using Anti-CANX antibody HPA009433 (A) shows similar protein distribution across tissues to independent antibody HPA009696 (B).
Product group Antibodies
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Anti-CANX AntibodyHPA009433
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetCANX
  • SizePrice
Immunohistochemical staining of human cerebral cortex, placenta, testis and tonsil using Anti-CANX antibody HPA009433 (A) shows similar protein distribution across tissues to independent antibody HPA009696 (B).
Product group Antibodies
Atlas Antibodies
Anti-CANX AntibodyHPA009433
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
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