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Anti-CARD10 Antibody

144-66373
RayBiotech
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCARD10
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-CARD10 Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Conjugate
    Unconjugated
  • Gene ID29775
  • Target name
    CARD10
  • Target description
    caspase recruitment domain family member 10
  • Target synonyms
    BIMP1, CARMA3, IMD89, caspase recruitment domain-containing protein 10, Bcl10 binding protein and activator of NFKB, CARD-containing MAGUK 3 protein, CARD-containing MAGUK protein 3, carma 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BWT7
  • Protein Name
    Caspase recruitment domain-containing protein 10
  • Scientific Description
    CARD10 pAb
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Figure 1. Western blot analysis of CARD10 using anti-CARD10 antibody (A07686-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD10 antigen affinity purified polyclonal antibody (A07686-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD10 at approximately 116 kDa. The expected band size for CARD10 is at 116 kDa.
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