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Western Blot analysis of 293 cells using CARD 10 Polyclonal Antibody
Western Blot analysis of 293 cells using CARD 10 Polyclonal Antibody
Western Blot analysis of 293 cells using CARD 10 Polyclonal Antibody

CARD10 Antibody

CSB-PA001213
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetCARD10
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Overview

  • Supplier
    Cusabio
  • Product Name
    CARD10 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID29775
  • Target name
    CARD10
  • Target description
    caspase recruitment domain family member 10
  • Target synonyms
    Bcl10 binding protein and activator of NFKB; BIMP1; CARD-containing MAGUK 3 protein; CARD-containing MAGUK protein 3; carma 3; CARMA3; caspase recruitment domain-containing protein 10
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9BWT7
  • Protein Name
    Caspase recruitment domain-containing protein 10
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CARD10 using anti-CARD10 antibody (A07686-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human Hacat whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD10 antigen affinity purified polyclonal antibody (A07686-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD10 at approximately 116 kDa. The expected band size for CARD10 is at 116 kDa.
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