Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111222/GTX111222_44378_20230303_WB_shRNA_watermark_23030717_278.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111222/GTX111222_44636_20230421_WB_23042500_429.webp "Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![CARD10 antibody [N2C1], Internal detects CARD10 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min](https://www.genetex.com/upload/website/prouct_img/normal/GTX111222/GTX111222_40058_20141128_IHC_w_23060500_416.webp "CARD10 antibody [N2C1], Internal detects CARD10 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:250.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min")
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111222/GTX111222_44378_20210820_WB_TPM_watermark_w_23060500_336.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.")
![CARD10 antibody [N2C1], Internal detects CARD10 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CARD10 stained by CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX111222/GTX111222_44636_20220422_IHC-P_M_w_23060500_199.webp "CARD10 antibody [N2C1], Internal detects CARD10 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. CARD10 stained by CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [N2C1], Internal (GTX111222) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CARD10 antibody [N2C1], Internal
GTX111222
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetCARD10
Overview
- SupplierGeneTex
- Product NameCARD10 antibody [N2C1], Internal
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.25 mg/ml
- ConjugateUnconjugated
- Gene ID29775
- Target nameCARD10
- Target descriptioncaspase recruitment domain family member 10
- Target synonymsBIMP1, CARMA3, IMD89, caspase recruitment domain-containing protein 10, Bcl10 binding protein and activator of NFKB, CARD-containing MAGUK 3 protein, CARD-containing MAGUK protein 3, carma 3
- HostRabbit
- IsotypeIgG
- Protein IDQ9BWT7
- Protein NameCaspase recruitment domain-containing protein 10
- Scientific DescriptionThe caspase recruitment domain (CARD) is a protein module that consists of 6 or 7 antiparallel alpha helices. It participates in apoptosis signaling through highly specific protein-protein homophilic interactions. Like several other CARD proteins, CARD10 belongs to the membrane-associated guanylate kinase (MAGUK) family and activates NF-kappa-B (NFKB; see MIM 164011) through BCL10 (MIM 603517) (Wang et al., 2001 [PubMed 11259443]).[supplied by OMIM]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- McAuley JR, Bailey KM, Ekambaram P, et al. MALT1 is a critical mediator of PAR1-driven NF-κB activation and metastasis in multiple tumor types. Oncogene. 2019,38(49):7384-7398. doi: 10.1038/s41388-019-0958-4Read this paper
- Kahle JJ, Gulbahce N, Shaw CA, et al. Comparison of an expanded ataxia interactome with patient medical records reveals a relationship between macular degeneration and ataxia. Hum Mol Genet. 2011,20(3):510-27. doi: 10.1093/hmg/ddq496Read this paper
Datasheet
Related products
Product group Antibodies
Anti-CARD10 Antibody144-66373
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetCARD10
- SizePrice
Product group Antibodies
CARD10 AntibodyCSB-PA001213
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetCARD10
- SizePrice
Product group Antibodies
Anti-CARD10 AntibodyA97094
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityHuman, Mouse
- SizePrice
Product group Antibodies
CARD10 antibodyGTX31678
ApplicationsELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetCARD10
- SizePrice
Product group Antibodies
Anti-CARD10 AntibodyHPA055612
ApplicationsImmunoCytoChemistry
ReactivityHuman
TargetCARD10
- SizePrice
Product group Antibodies
CARD10 / CARMA3 AntibodyLS-C349097
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetCARD10
- SizePrice
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [HL2260] (GTX638312) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638312/GTX638312_T-44970_20230317_WB_TPM_watermark_23032022_559.webp)
Product group Antibodies
CARD10 antibody [HL2260]GTX638312
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetCARD10
- SizePrice
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CARD10 antibody [HL2261] (GTX638313) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX638313/GTX638313_T-44970_20230303_WB_shRNA_watermark_23030717_854.webp)
Product group Antibodies
CARD10 antibody [HL2261]GTX638313
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetCARD10
- SizePrice
Product group Antibodies
Anti-CARD10 Antibody Picoband(r)A07686-4-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetCARD10
- SizePrice