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Figure 1. Western blot analysis of CARD12 using anti-CARD12 antibody (PB9673). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: 22RV1 Whole Cell Lysate, Lane 2: RH35 Whole Cell Lysate, Lane 3: NIH3T3 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD12 antigen affinity purified polyclonal antibody (Catalog # PB9673) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD12 at approximately 116 kDa. The expected band size for CARD12 is at 116 kDa.
Figure 1. Western blot analysis of CARD12 using anti-CARD12 antibody (PB9673). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: 22RV1 Whole Cell Lysate, Lane 2: RH35 Whole Cell Lysate, Lane 3: NIH3T3 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD12 antigen affinity purified polyclonal antibody (Catalog # PB9673) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD12 at approximately 116 kDa. The expected band size for CARD12 is at 116 kDa.
Figure 1. Western blot analysis of CARD12 using anti-CARD12 antibody (PB9673). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 40 ug of sample under reducing conditions. Lane 1: 22RV1 Whole Cell Lysate, Lane 2: RH35 Whole Cell Lysate, Lane 3: NIH3T3 Whole Cell Lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CARD12 antigen affinity purified polyclonal antibody (Catalog # PB9673) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CARD12 at approximately 116 kDa. The expected band size for CARD12 is at 116 kDa.

Anti-CARD12/NLRC4 Antibody Picoband(r)

PB9673-DYLIGHT550
Boster Bio
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetNLRC4
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-CARD12/NLRC4 Antibody Picoband(r)
  • Delivery Days Customer
    9
  • Antibody Specificity
    No cross reactivity with other proteins.
  • Application Supplier Note
    Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Conjugate
    DyLight 550
  • Gene ID58484
  • Target name
    NLRC4
  • Target description
    NLR family CARD domain containing 4
  • Target synonyms
    AIFEC; CARD, LRR, and NACHT-containing protein; CARD12; caspase recruitment domain family, member 12; caspase recruitment domain-containing protein 12; CED-4-like protein Clan; CLAN; CLAN1; CLANA; CLANB; CLANC; CLAND; CLR2.1; FCAS4; ICE-protease activating factor; IPAF; NLR family CARD domain-containing protein 4; NOD-like receptor C4; nucleotide-binding oligomerization domain, leucine rich repeat and CARD domain containing 4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9NPP4
  • Protein Name
    NLR family CARD domain-containing protein 4
  • Scientific Description
    Boster Bio Anti-CARD12/NLRC4 Antibody Picoband® catalog # PB9673. Tested in IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203