Western blot analysis using anti-caspase-2, mAb (11B4) (Prod. No. AG-20T-0136), detecting caspase-2 expression in cultured mouse and human cell lines. Caspase-2 protein was revealed in lysates from 2x105 mouse and human cell lines by 11B4 and E
Western blot analysis using anti-caspase-2, mAb (11B4) (Prod. No. AG-20T-0136), detecting caspase-2 expression in cultured mouse and human cell lines. Caspase-2 protein was revealed in lysates from 2x105 mouse and human cell lines by 11B4 and E
anti-Caspase-2, mAb (11B4)
AG-20T-0136
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Mouse, Rat
TargetCASP2
Overview
- SupplierAdipoGen Life Sciences
- Product Nameanti-Caspase-2, mAb (11B4)
- Delivery Days Customer10
- Antibody SpecificityRecognizes an epitope in the p19 subunit of human, mouse, rat, monkey and dog caspase-2. Detects bands of ~51kDa (procaspase-2), ~32kDa and ~18kDa (cleavage products) by Western blot.
- ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID11B4
- Concentration1 mg/ml
- Estimated Purity>95%
- FormulationLiquid
- Gene ID835
- Target nameCASP2
- Target descriptioncaspase 2
- Target synonymsCASP-2; caspase 2 apoptosis-related cysteine peptidase; caspase-2; ICH1; NEDD2; NEDD-2; neural precursor cell expressed developmentally down-regulated protein 2; PPP1R57; protease ICH-1; protein phosphatase 1, regulatory subunit 57
- HostRat
- IsotypeIgG2a
- Protein IDP42575
- Protein NameCaspase-2
- Scientific DescriptionCaspase-2 is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved into smaller fragments. Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe and is involved in the activation cascade of caspases responsible for apoptosis execution. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD. This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events. In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase-3 activation, extending a possible role for caspase-2 as a parallel effector caspase. - Monoclonal Antibody. Recognizes an epitope in the p19 subunit of human, mouse, rat, monkey and dog caspase-2. Detects bands of ~51kDa (procaspase-2), ~32kDa and ~18kDa (cleavage products) by Western blot. Isotype: Rat IgG2akappa. Clone: 11B4. Applications: ELISA, FACS, ICC, IHC, IP, WB. Liquid. In PBS containing 0.02% sodium azide. Caspase-2 is a Class I caspase with a long prodomain necessary for nuclear localization. Upon activation of the apoptotic pathway, the procaspase is cleaved into smaller fragments. Caspase-2 is the nuclear apoptotic respondent to cellular genotoxic stress or mitotic catastrophe and is involved in the activation cascade of caspases responsible for apoptosis execution. Activation occurs upon recruitment to a complex containing a p53-induced death domain protein, PIDD. This suggests caspase-2 can be a nuclear initiator caspase with a requirement for caspase-9 and caspase-3 activation in downstream apoptotic events. In apoptotic pathways resulting from UV-induced DNA damage, processing of caspase-2 occurs downstream of mitochondrial dysfunction and of caspase-9 and caspase-3 activation, extending a possible role for caspase-2 as a parallel effector caspase.
- ReactivityCanine, Human, Monkey, Mouse, Rat
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203