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Immunofluorescent staining of human cell line HAP1 shows localization to plasma membrane.
Immunofluorescent staining of human cell line HAP1 shows localization to plasma membrane.
Immunofluorescent staining of human cell line HAP1 shows localization to plasma membrane.

Anti-CCKBR Antibody

HPA079548
Atlas Antibodies
ApplicationsImmunoCytoChemistry
DownloadMSDS
Product group Antibodies
ReactivityHuman
TargetCCKBR
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CCKBR
  • Delivery Days Customer
    12
  • Applications
    ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID887
  • Target name
    CCKBR
  • Target description
    cholecystokinin B receptor
  • Target synonyms
    CCK2 receptor; CCK2R; CCK-B; CCK-B receptor; cholecystokinin-2 receptor; GASR; gastrin receptor; gastrin/cholecystokinin type B receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32239
  • Protein Name
    Gastrin/cholecystokinin type B receptor
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CCKBR using anti-CCKBR antibody (A01677-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCKBR antigen affinity purified polyclonal antibody (Catalog # A01677-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCKBR at approximately 64KD. The expected band size for CCKBR is at 48KD.
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