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Mouse Stomach lysates probed with CCKBR Polyclonal Antibody, Unconjugated (bs-1777R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse Stomach lysates probed with CCKBR Polyclonal Antibody, Unconjugated (bs-1777R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Mouse Stomach lysates probed with CCKBR Polyclonal Antibody, Unconjugated (bs-1777R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.

CCKBR Polyclonal Antibody

BS-1777R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Human, Mouse, Rabbit, Rat
TargetCCKBR
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Overview

  • Supplier
    Bioss
  • Product Name
    CCKBR Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID887
  • Target name
    CCKBR
  • Target description
    cholecystokinin B receptor
  • Target synonyms
    CCK-2R, CCK-B, CCK2R, GASR, gastrin/cholecystokinin type B receptor, CCK-B receptor, CCK2 receptor, cholecystokinin type 2 receptor, cholecystokinin-2 receptor, gastrin receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32239
  • Protein Name
    Gastrin/cholecystokinin type B receptor
  • Reactivity
    Bovine, Human, Mouse, Rabbit, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Mohammad S, Ozaki T, Takeuchi K, et al. Functional compensation between cholecystokinin-1 and -2 receptors in murine paraventricular nucleus neurons. J Biol Chem. 2012,287(47):39391-401. doi: 10.1074/jbc.M112.416214
    Read this paper
  • Kalsheker NA, Hodgson IJ, Watkins GL, et al. Deoxyribonucleic acid (DNA) polymorphism of the alpha 1-antitrypsin gene in chronic lung disease. Br Med J (Clin Res Ed). 1987,294(6586):1511-4.
    Read this paper

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Figure 1. Western blot analysis of CCKBR using anti-CCKBR antibody (A01677-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCKBR antigen affinity purified polyclonal antibody (Catalog # A01677-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCKBR at approximately 64KD. The expected band size for CCKBR is at 48KD.
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