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Western Blot analysis of K562 cells using CCK-BR Polyclonal Antibody
Western Blot analysis of K562 cells using CCK-BR Polyclonal Antibody
Western Blot analysis of K562 cells using CCK-BR Polyclonal Antibody

CCKBR Antibody

CSB-PA001401
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetCCKBR
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Overview

  • Supplier
    Cusabio
  • Product Name
    CCKBR Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID887
  • Target name
    CCKBR
  • Target description
    cholecystokinin B receptor
  • Target synonyms
    CCK-2R, CCK-B, CCK2R, GASR, gastrin/cholecystokinin type B receptor, CCK-B receptor, CCK2 receptor, cholecystokinin type 2 receptor, cholecystokinin-2 receptor, gastrin receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP32239
  • Protein Name
    Gastrin/cholecystokinin type B receptor
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CCKBR using anti-CCKBR antibody (A01677-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human PANC-1 whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human COLO-320 whole cell lysates, Lane 4: human SGC-7901 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCKBR antigen affinity purified polyclonal antibody (Catalog # A01677-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCKBR at approximately 64KD. The expected band size for CCKBR is at 48KD.
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