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Figure 1. Western blot analysis of CCL2 using anti-CCL2 antibody (A00056-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL2 antigen affinity purified polyclonal antibody (Catalog # A00056-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL2 at approximately 11KD. The expected band size for CCL2 is at 11KD.
Figure 1. Western blot analysis of CCL2 using anti-CCL2 antibody (A00056-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL2 antigen affinity purified polyclonal antibody (Catalog # A00056-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL2 at approximately 11KD. The expected band size for CCL2 is at 11KD.
Figure 1. Western blot analysis of CCL2 using anti-CCL2 antibody (A00056-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL2 antigen affinity purified polyclonal antibody (Catalog # A00056-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL2 at approximately 11KD. The expected band size for CCL2 is at 11KD.

Anti-CCL2 Antibody

A00056-4
Boster Bio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse, Rat
TargetCcl2
100 ug
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-CCL2 Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    500 ug/ml
  • Gene ID24770
  • Target name
    Ccl2
  • Target description
    C-C motif chemokine ligand 2
  • Target synonyms
    MCP-1, Scya2, Sigje, C-C motif chemokine 2, chemokine (C-C motif) ligand 2, immediate-early serum-responsive JE protein, immediate-early serum-responsive protein JE, monocyte chemoattractant protein 1, monocyte chemotactic protein 1, small inducible cytokine A2, small inducible gene JE
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14844
  • Protein Name
    C-C motif chemokine 2
  • Scientific Description
    Boster Bio Anti-CCL2 Antibody Picoband® catalog # A00056-4. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Shi H, Duan X, Dong J, et al. RNA-seq combined network pharmacology reveals that Fu-Gan-Wan (FGW) inhibits liver fibrosis via NF-κB/CCL2/CCR2 and lipid peroxidation via Nrf2/HMOX1 signaling pathway. J Ethnopharmacol. 2024,326:117963. doi: 10.1016/j.jep.2024.117963
    Read this paper
  • Xie S, Su E, Song X, et al. GSDME in Endothelial Cells: Inducing Vascular Inflammation and Atherosclerosis via Mitochondrial Damage and STING Pathway Activation. Biomedicines. 2023,11(9). doi: 10.3390/biomedicines11092579
    Read this paper
  • Zhang BL, Yu P, Su EY, et al. Inhibition of GSDMD activation by Z-LLSD-FMK or Z-YVAD-FMK reduces vascular inflammation and atherosclerotic lesion development in ApoE(-/-) mice. Front Pharmacol. 2023,14:1184588. doi: 10.3389/fphar.2023.1184588
    Read this paper
  • Fang H, Yang T, Zhou B, et al. (Pro)Renin Receptor Decoy Peptide PRO20 Protects against Oxidative Renal Damage Induced by Advanced Oxidation Protein Products. Molecules. 2023,28(7). doi: 10.3390/molecules28073017
    Read this paper
  • Šešelja K, Bazina I, Welss J, et al. Effect of Tff3 Deficiency and ER Stress in the Liver. Int J Mol Sci. 2019,20(18). doi: 10.3390/ijms20184389
    Read this paper
  • Tian Y, Li Y, Li J, et al. Bufei Yishen Granules Combined with Acupoint Sticking Therapy Suppress Inflammation in Chronic Obstructive Pulmonary Disease Rats: Via JNK/p38 Signaling Pathway. Evid Based Complement Alternat Med. 2017,2017:1768243. doi: 10.1155/2017/1768243
    Read this paper
  • Wang XT, Gong Y, Zhou B, et al. Ursolic acid ameliorates oxidative stress, inflammation and fibrosis in diabetic cardiomyopathy rats. Biomed Pharmacother. 2018,97:1461-1467. doi: 10.1016/j.biopha.2017.11.032
    Read this paper
  • Zhou J, Jin J, Li X, et al. Total flavonoids of Desmodium styracifolium attenuates the formation of hydroxy-L-proline-induced calcium oxalate urolithiasis in rats. Urolithiasis. 2018,46(3):231-241. doi: 10.1007/s00240-017-0985-y
    Read this paper
  • Li L, Bian T, Lyu J, et al. Human β-defensin-3 alleviates the progression of atherosclerosis accelerated by Porphyromonas gingivalis lipopolysaccharide. Int Immunopharmacol. 2016,38:204-13. doi: 10.1016/j.intimp.2016.06.003
    Read this paper
  • Bai X, Qi Z, Song G, et al. Effects of Monocyte Chemotactic Protein-1 and Nuclear Factor of Kappa B Pathway in Rejection of Cardiac Allograft in Rat. Transplant Proc. 2015,47(6):2010-6. doi: 10.1016/j.transproceed.2015.05.014
    Read this paper

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Figure 1. Western blot analysis of CCL2 using anti-CCL2 antibody (A00056-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCL2 antigen affinity purified polyclonal antibody (Catalog # A00056-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CCL2 at approximately 11KD. The expected band size for CCL2 is at 11KD.
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