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Anti-CD10 [60656-7H5]

Ab03240-10.3
Absolute Antibody
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetMME
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD10 [60656-7H5]
  • Delivery Days Customer
    7
  • Application Supplier Note
    The antibody detected the recombinant CD10 by western blot analysis. The antibody was used to stain and analyze the renal clear cell carcinoma tissue by immunohistochemistry. Further, immunohistochemistry staining on renal clear cell carcinoma tissue from 60656-7H5 antibody and the commercial antibody, 56C6, were compared, showing the staining intensity of 60656-7H5 antibody was higher and the specificity was consistent (CN103880960).
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Applications Supplier
    IHC; WB; ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    60656-7H5
  • Gene ID4311
  • Target name
    MME
  • Target description
    membrane metalloendopeptidase
  • Target synonyms
    CALLA, CD10, CMT2T, NEP, SCA43, SFE, neprilysin, atriopeptidase, common acute lymphocytic leukemia antigen, membrane metallo-endopeptidase (neutral endopeptidase, enkephalinase, CALLA, CD10), neprilysin-390, neprilysin-411, neutral endopeptidase 24.11, skin fibroblast elastase
  • Host
    Human
  • Isotype
    IgG1
  • Protein IDP08473
  • Protein Name
    Neprilysin
  • Scientific Description
    This chimeric human antibody was made using the variable domain sequences of the original Mouse IgG2A format for improved compatibility with existing reagents assays and techniques.
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    The original antibody was obtained by immunizing mice with recombinant CD10 protein.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD10/MME using anti-CD10/MME antibody (PB9058). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Daudi whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: rat kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD10/MME antigen affinity purified polyclonal antibody (Catalog # PB9058) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD10/MME at approximately 100 kDa. The expected band size for CD10/MME is at 85 kDa.
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