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ProductsBack/CD133 antibody [3F10]
IHC-P analysis of human breast cancer tissues (left) and human esophageal cancer tissues (right) using GTX60471 CD133 antibody [3F10]
IHC-P analysis of human breast cancer tissues (left) and human esophageal cancer tissues (right) using GTX60471 CD133 antibody [3F10]
IHC-P analysis of human breast cancer tissues (left) and human esophageal cancer tissues (right) using GTX60471 CD133 antibody [3F10]

CD133 antibody [3F10]

GTX60471
GeneTex
ApplicationsFlow Cytometry, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPROM1
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD133 antibody [3F10]
  • Delivery Days Customer
    9
  • Application Supplier Note
    IHC-P: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3F10
  • Conjugate
    Unconjugated
  • Gene ID8842
  • Target name
    PROM1
  • Target description
    prominin 1
  • Target synonyms
    AC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4, prominin-1, AC133 antigen, Retinitis pigmentosa 41, Cone-rod dystrophy 12, antigen AC133, hProminin, hematopoietic stem cell antigen, prominin-like protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO43490
  • Protein Name
    Prominin-1
  • Scientific Description
    This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Kim J, Shin K, Lee SH, et al. Slug and CD133 expression are associated with peritoneal carcinomatosis and survival in gastric cancer. J Gastrointest Oncol. 2021,12(4):1326-1337. doi: 10.21037/jgo-21-123
    Read this paper
  • Luna ECM, Bezerra TMM, Barros Silva PG, et al. CD133 Role in Oral Carcinogenesis. Asian Pac J Cancer Prev. 2020,21(9):2501-2506. doi: 10.31557/APJCP.2020.21.9.2501
    Read this paper

Datasheet

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Figure 1. Western blot analysis of PROM1 using anti-PROM1 antibody (A01767-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human CACO-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PROM1 antigen affinity purified polyclonal antibody (Catalog # A01767-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PROM1 at approximately 97KD. The expected band size for PROM1 is at 97KD.
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