![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (GTX636673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (GTX636673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636673/GTX636673_44599_20220225_WB_w_23061202_503.webp)
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (GTX636673) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CD133 antibody [HL1271]
GTX636673
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetPROM1
Overview
- SupplierGeneTex
- Product NameCD133 antibody [HL1271]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsWestern Blot
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDHL1271
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID8842
- Target namePROM1
- Target descriptionprominin 1
- Target synonymsAC133, CD133, CORD12, MCDR2, MSTP061, PROML1, RP41, STGD4, prominin-1, AC133 antigen, Retinitis pigmentosa 41, Cone-rod dystrophy 12, antigen AC133, hProminin, hematopoietic stem cell antigen, prominin-like protein 1
- HostRabbit
- IsotypeIgG
- Protein IDO43490
- Protein NameProminin-1
- Scientific DescriptionThis gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (GTX636673) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CD133 antibody [HL1271] (GTX636673) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX636673/GTX636673_T-44536_20220121_WB_B_w_23061202_855.webp)


![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD133 antibody [C1C2], Internal (GTX100567) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX100567/GTX100567_39471_20190530_WB_w_23060100_319.webp)
![Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with CD133 antibody [N1C1] (GTX102109) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.](https://www.genetex.com/upload/website/prouct_img/normal/GTX102109/GTX102109_39549_20190530_WB_w_23060100_233.webp)
![IHC-P analysis of human breast cancer tissues (left) and human esophageal cancer tissues (right) using GTX60471 CD133 antibody [3F10]](https://www.genetex.com/upload/website/prouct_img/normal/GTX60471/GTX60471_20170912_IHC-P_w_23061123_823.webp)
![CD133 antibody [HL1631] (GTX637124) detects CD133 protein by flow cytometry analysis. Sample: NT2D1 cell. Gray: Rabbit IgG Isotype control. Red: CD133 antibody [HL1631] (GTX637124) dilution: 1:50. Acquisition of 20,000 events were collected for FACS analysis. *The competitor (clone [AC133]) is not affiliated with GeneTex and does not endorse this product.](https://www.genetex.com/upload/website/prouct_img/normal/GTX637124/GTX637124_T-44739_20220722_FACS_22081000_264.webp)