Anti-CD134 [9D1]

Absolute Antibody

Anti-CD134 [9D1]

7

This antibody binds human CD134 (OX40; TNFRFS4). This molecule acts as a receptor for TNFSF4/OX40L/GP34. It is a costimulatory molecule implicated in long-term T-cell immunity.

The binding characterization of this antibody to human OX40 was done using ELISA. The binding reactivity of this antibody to human, mouse and rhesus monkey OX40 expressed on CHO cells was determined using flow cytometry. This antibody was shown to cross react with human and rhesus monkey OX40 and not mouse OX40. The binding affinity of the original mouse IgG1 antibody for human OX40 was determined using surface plasmon resonance and was found to be Kd= 0.389 nM. The anti-tumor activity of this antibody was studied in an in vivo mouse colon cancer model. MC-38 cancer tumor cells (colon adenocarcinoma cells) were injected subcutaneously into B-hOX40 humanized mice and the mice were then treated with this antibody. This antibody was shown to have good percent tumor growth inhibition (CN111763258).

Flow Cytometry, ELISA

ELISA; FC; therapeutic

Research Use Only

Monoclonal

9D1

TNFRSF4

TNF receptor superfamily member 4

ACT35; ACT35 antigen; ATC35 antigen; CD134; CD134 antigen; IMD16; lymphoid activation antigene ACT35; OX40; OX40 antigen; OX40 cell surface antigen; OX40 homologue; OX40L receptor; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1 receptor; tumor necrosis factor receptor superfamily member 4; TXGP1L

Rabbit

IgG

Tumor necrosis factor receptor superfamily member 4

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.

Human, Primate

Human, rhesus monkey

The original antibody was generated by immunizing 6-8 weeks old female BALB/c mice with human OX40 protein.

-20°C,2°C to 8°C

12352203