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Western blot analysis of CD134 expression in Human fetal spleen lysate.
Western blot analysis of CD134 expression in Human fetal spleen lysate.
Western blot analysis of CD134 expression in Human fetal spleen lysate.

Anti-CD134 Monoclonal Antibody

M02495
Boster Bio
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
ReactivityHuman
TargetTNFRSF4
100 ul
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Overview

  • Supplier
    Boster Bio
  • Product Name
    Anti-CD134 Monoclonal Antibody
  • Delivery Days Customer
    9
  • Applications
    Flow Cytometry, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AEHA-20
  • Gene ID7293
  • Target name
    TNFRSF4
  • Target description
    TNF receptor superfamily member 4
  • Target synonyms
    ACT35, CD134, IMD16, OX40, TXGP1L, tumor necrosis factor receptor superfamily member 4, ACT35 antigen, ATC35 antigen, CD134 antigen, OX40 antigen, OX40 cell surface antigen, OX40 homologue, OX40L receptor, TAX transcriptionally-activated glycoprotein 1 receptor, lymphoid activation antigene ACT35, tax-transcriptionally activated glycoprotein 1 receptor
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP43489
  • Protein Name
    Tumor necrosis factor receptor superfamily member 4
  • Scientific Description
    Boster Bio Anti-CD134 Monoclonal Antibody catalog # M02495. Tested in WB, Flow Cytometry applications. This antibody reacts with Human.
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

Datasheet

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MSDS

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Figure 1. Western blot analysis of CD134 using anti-CD134 antibody (PB9898). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates, Lane 2: 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134 antigen affinity purified polyclonal antibody (Catalog # PB9898) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134 at approximately 50KD. The expected band size for CD134 is at 50KD.
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