Anti-CD166 [EJ212/007-C12-5]

AB02669-10.3-BT
ApplicationsFunctional Assay, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetALCAM
Overview
- SupplierAbsolute Antibody
- Product NameAnti-CD166 [EJ212/007-C12-5]
- Delivery Days Customer9
- Antibody SpecificityThis antibody is specific for human CD166. CD166 is a protein found in cancer tissues. CD166 is also cell adhesion molecule that mediates both heterotypic cell-cell contacts via its interaction with CD6, as well as homotypic cell-cell contacts. Promotes T
- Application Supplier NoteThe ability of the scFv version of this antibody to bind to PM-1, PBL and granulocytes was assayed by ELISA and flow cytometry. This confirmed that it only bound PM-1 (CA2682059A1). Futhermore the selectivity of the scFv version of this antibody was tested on human tumor tissues including breast, prostate and kidney tumors from 11-14 different donors. breast, prostate and kidney tumors showed the strongest binding. Then to test the specificty of tumors versus normal tissue a tissue microarray with 13 human breast tumor tissues and to a tissue microarray with 4 normal breast tissues using immunohistochemistry staining was preformed with the scFv version of this antibody. 6/13 tumors showed specific staining and none of the normal tissues showed any staining (CA2682059A1). To capture the antigen to which this antibody binds, immunoprecipitation was preformed with the human version of this antibody (CA2682059A1). Futhermore the immuoprecipitated protein was blotted using the human version of this antibody (CA2682059A1). To evaluate the activity of the human version of this antibody as an inductor of apoptosis the effect of the antibody on BT474 was studied. I did indeed induce apoptosis. (CA2682059A1). By detecting the scFv version of this antibody with a secondary antibody it was determined that PM-1 cells showed some internalization of this antibody (CA2682059A1).
- ApplicationsFunctional Assay, ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDEJ212/007-C12-5
- Gene ID214
- Target nameALCAM
- Target descriptionactivated leukocyte cell adhesion molecule
- Target synonymsCD166; CD166 antigen; MEMD
- HostHuman
- IsotypeIgG1
- Protein IDQ13740
- Protein NameCD166 antigen
- ReactivityHuman
- Storage Instruction-20°C,2°C to 8°C
- UNSPSC12352203