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Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.

Anti-CD1B Antibody

HPA021824
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCD1B
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Overview

  • Supplier
    Atlas Antibodies
  • Product Name
    Anti-CD1B
  • Delivery Days Customer
    4
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID910
  • Target name
    CD1B
  • Target description
    CD1b molecule
  • Target synonyms
    CD1, CD1A, R1, T-cell surface glycoprotein CD1b, CD1B antigen, b polypeptide, cortical thymocyte antigen CD1B, differentiation antigen CD1-alpha-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29016
  • Protein Name
    T-cell surface glycoprotein CD1b
  • Scientific Description
    Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

Datasheet

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MSDS

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Figure 1. Western blot analysis of CD1b using anti-CD1b antibody (A02158-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysate, Lane 3: human SW620 whole cell lysate, Lane 4: human THP-1 whole cell lysate, Lane 5: human MDA-MB-231 whole cell lysate, Lane 6: human K562 whole cell lysate, Lane 7: human A431 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1b antigen affinity purified polyclonal antibody (Catalog # A02158-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1b at approximately 65KD. The expected band size for CD1b is at 37KD.
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