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Anti-CD1B Antibody

CAB6551
Assay Genie
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetCD1B
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Overview

  • Supplier
    Assay Genie
  • Product Name
    Anti-CD1B Antibody
  • Delivery Days Customer
    9
  • Applications
    Western Blot, ELISA
  • Applications Supplier
    WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Not applicable
  • Conjugate
    Unconjugated
  • Gene ID910
  • Target name
    CD1B
  • Target description
    CD1b molecule
  • Target synonyms
    CD1, CD1A, R1, T-cell surface glycoprotein CD1b, CD1B antigen, b polypeptide, cortical thymocyte antigen CD1B, differentiation antigen CD1-alpha-3
  • Host
    Rabbit
  • Protein IDP29016
  • Protein Name
    T-cell surface glycoprotein CD1b
  • Scientific Description
    CD1B Antibody is a premium polyclonal antibody that offers outstanding performance and reliability for demanding research applications. Rigorously validated for WB, ELISA, this antibody ensures consistent, reproducible results across multiple experimental platforms. Demonstrates excellent reactivity with Human samples, providing researchers with confidence in cross-species compatibility. Conveniently packaged in 100microL format to meet your experimental needs. For optimal performance, store at -20°C and maintains stability for 12 months. Backed by rigorous quality control testing to ensure superior performance in your critical research applications.
  • Shelf life instruction
    12 months
  • Source
    Rabbit
  • Reactivity
    Human
  • Reactivity Supplier
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD1b using anti-CD1b antibody (A02158-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysate, Lane 3: human SW620 whole cell lysate, Lane 4: human THP-1 whole cell lysate, Lane 5: human MDA-MB-231 whole cell lysate, Lane 6: human K562 whole cell lysate, Lane 7: human A431 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1b antigen affinity purified polyclonal antibody (Catalog # A02158-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1b at approximately 65KD. The expected band size for CD1b is at 37KD.
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