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CD1b Monoclonal Antibody

BSM-60395M
Bioss Antibodies
ApplicationsFlow Cytometry
Product group Antibodies
TargetCD1B
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    CD1b Monoclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry
  • Applications Supplier
    FCM(1:20-100)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    E2E2.
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID910
  • Target name
    CD1B
  • Target description
    CD1b molecule
  • Target synonyms
    CD1; CD1A; CD1B antigen, b polypeptide; cortical thymocyte antigen CD1B; differentiation antigen CD1-alpha-3; R1; T-cell surface glycoprotein CD1b
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP29016
  • Protein Name
    T-cell surface glycoprotein CD1b
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD1b using anti-CD1b antibody (A02158-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human PC-3 whole cell lysate, Lane 3: human SW620 whole cell lysate, Lane 4: human THP-1 whole cell lysate, Lane 5: human MDA-MB-231 whole cell lysate, Lane 6: human K562 whole cell lysate, Lane 7: human A431 whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1b antigen affinity purified polyclonal antibody (Catalog # A02158-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1b at approximately 65KD. The expected band size for CD1b is at 37KD.
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