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Anti-CD1c [M241]

AB00820-1.1-BT
Absolute Antibody
ApplicationsImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry, RadioImmunoAssay
Product group Antibodies
TargetCD1C
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-CD1c [M241]
  • Delivery Days Customer
    9
  • Application Supplier Note
    M241 has been used to characterise B cells in severe combined immunodeficiency disease by immunofluorescence (Small TN et al 1989).
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry, RadioImmunoAssay
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    M241
  • Gene ID911
  • Target name
    CD1C
  • Target description
    CD1c molecule
  • Target synonyms
    BDCA1; CD1; CD1A; CD1C antigen, c polypeptide; cortical thymocyte antigen CD1C; differentiation antigen CD1-alpha-3; R7; T-cell surface glycoprotein CD1c
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP29017
  • Protein Name
    T-cell surface glycoprotein CD1c
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD1c using anti-CD1c antibody (A01188-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1c antigen affinity purified polyclonal antibody (Catalog # A01188-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1c at approximately 62KD. The expected band size for CD1c is at 38KD.
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