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CD1c antibody

GTX18216
GeneTex
ApplicationsImmunoPrecipitation, Other Application
Product group Antibodies
TargetCD1C
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Overview

  • Supplier
    GeneTex
  • Product Name
    CD1c antibody [M241]
  • Delivery Days Customer
    9
  • Application Supplier Note
    IP: Use at an assay dependent dilution. RID: Use at an assay dependent dilution. Optimal dilutions/concentrations should be determined by the end user.
  • Applications
    ImmunoPrecipitation, Other Application
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID911
  • Target name
    CD1C
  • Target description
    CD1c molecule
  • Target synonyms
    BDCA1; CD1; CD1A; CD1C antigen, c polypeptide; cortical thymocyte antigen CD1C; differentiation antigen CD1-alpha-3; R7; T-cell surface glycoprotein CD1c
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP29017
  • Protein Name
    T-cell surface glycoprotein CD1c
  • Scientific Description
    This gene encodes a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by this gene is broadly distributed throughout the endocytic system via a tyrosine-based motif in the cytoplasmic tail. Alternatively spliced transcript variants of this gene have been observed, but their full-length nature is not known. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD1c using anti-CD1c antibody (A01188-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1c antigen affinity purified polyclonal antibody (Catalog # A01188-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1c at approximately 62KD. The expected band size for CD1c is at 38KD.
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