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Western blot All lanes: CD1C antibody at 3.89ug/ml Lane 1: HepG2 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa
Western blot All lanes: CD1C antibody at 3.89ug/ml Lane 1: HepG2 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa
Western blot All lanes: CD1C antibody at 3.89ug/ml Lane 1: HepG2 whole cell lysate Lane 2: A549 whole cell lysate Lane 3: MCF-7 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 38 kDa Observed band size: 38 kDa

CD1C Antibody

CSB-PA004891ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetCD1C
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Overview

  • Supplier
    Cusabio
  • Product Name
    CD1C Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID911
  • Target name
    CD1C
  • Target description
    CD1c molecule
  • Target synonyms
    BDCA1, CD1, CD1A, R7, T-cell surface glycoprotein CD1c, CD1C antigen, c polypeptide, cortical thymocyte antigen CD1C, differentiation antigen CD1-alpha-3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP29017
  • Protein Name
    T-cell surface glycoprotein CD1c
  • Scientific Description
    Antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD1c using anti-CD1c antibody (A01188-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD1c antigen affinity purified polyclonal antibody (Catalog # A01188-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD1c at approximately 62KD. The expected band size for CD1c is at 38KD.
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