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ProductsBack/ITGB1 / Integrin Beta 1 / CD29 Antibody (clone TS2/16.2.1)

ITGB1 / Integrin Beta 1 / CD29 Antibody (clone TS2/16.2.1)

LS-C770003
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry
Product group Antibodies
TargetITGB1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    ITGB1 / Integrin Beta 1 / CD29 Antibody (clone TS2/16.2.1)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is less than or equal to 0.5 microg per 10E6 cells in 100 microl volume or 100 microl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.. Flo, IF, IHC, IP Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is less than or equal to 0.5 µg per 10E6 cells in 100 µl volume or 100 µl of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
  • Applications
    Flow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    TS2/16.2.1
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID3688
  • Target name
    ITGB1
  • Target description
    integrin subunit beta 1
  • Target synonyms
    CD29; FNRB; glycoprotein IIa; GPIIA; integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); MDF2; MSK12; very late activation protein, beta polypeptide; VLAB; VLA-BETA
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Integrin Beta 1/ITGB1 using anti-Integrin Beta 1/ITGB1 antibody (A00772-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT-1080 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Beta 1/ITGB1 antigen affinity purified polyclonal antibody (Catalog # A00772-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Beta 1/ITGB1 at approximately 88,115-140 kDa. The expected band size for Integrin Beta 1/ITGB1 is at 88 kDa.
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