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Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-Integrin beta 1/CD29 Polyclonal Antibody (bs-0486R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-Integrin beta 1/CD29 Polyclonal Antibody (bs-0486R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining
Formalin-fixed and paraffin embedded rat skin tissue labeled with Anti-Integrin beta 1/CD29 Polyclonal Antibody (bs-0486R), Unconjugated at 1:200, followed by conjugation to the secondary antibody and DAB staining

Integrin beta 1 Polyclonal Antibody

BS-0486R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetITGB1
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Integrin beta 1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3688
  • Target name
    ITGB1
  • Target description
    integrin subunit beta 1
  • Target synonyms
    CD29; FNRB; glycoprotein IIa; GPIIA; integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includes MDF2, MSK12); MDF2; MSK12; very late activation protein, beta polypeptide; VLAB; VLA-BETA
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP05556
  • Protein Name
    Integrin beta-1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • In vitro culture and biological properties of broiler adipose-derived stem cells. Lu T et al., 2018 Sep, Exp Ther Med
    Read more
  • MicroRNA 143-5p regulates alpaca melanocyte migration, proliferation and melanogenesis. Ji K et al., 2018 Feb, Exp Dermatol
    Read more
  • Loss of calponin h1 confers anoikis resistance and tumor progression in the development of high-grade serous carcinoma originating from the fallopian tube epithelium. Wang KH et al., 2017 Sep 22, Oncotarget
    Read more
  • Knockdown of Snail inhibits epithelial-mesenchymal transition of human laryngeal squamous cell carcinoma Hep-2 cells through the vitamin D receptor signaling pathway. Zhao X et al., 2017 Dec, Biochem Cell Biol
    Read more
  • Osteogenic Potential of the Transcription Factor c-MYB. Oralova V et al., 2017 Mar, Calcif Tissue Int
    Read more
  • Inositol Hexaphosphate and Inositol Inhibit Colorectal Cancer Metastasis to the Liver in BALB/c Mice. Fu M et al., 2016 May 12, Nutrients
    Read more

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Figure 1. Western blot analysis of Integrin Beta 1/ITGB1 using anti-Integrin Beta 1/ITGB1 antibody (A00772-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HT-1080 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human A431 whole cell lysates, Lane 4: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Integrin Beta 1/ITGB1 antigen affinity purified polyclonal antibody (Catalog # A00772-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Integrin Beta 1/ITGB1 at approximately 88,115-140 kDa. The expected band size for Integrin Beta 1/ITGB1 is at 88 kDa.
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